EDUCATIONAL BACKGROUND

Ph.D. (2001) Division of Clinical Bacteriology, Karolinska Institute, Sweden

B.Sc. (1995) Department of Biochemistry, Zhongshan University, P.R.China

PROFESSIONAL EXPERIENCE

Organisation for International Dialogue and Conflict Management, Biosafety Working Group, Austria. Postdoctoral Research Scientist since 06.2009

Unit for Molecular Glycobiology, Department of Molecular Biomedical Research Flanders Institute for Biotechnology (VIB), Department of Molecular Biology, Gent University (UGent), Gent-Zwijnaard, Belgium. Postdoctoral Research Scientist 10.2005-05.2009

BioHelix Inc./ New England Biolabs Inc., Beverly/ Ipswich, MA, USA. Postdoctoral Research Scientist 04.2005-09.2005

Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital/Harvard Medical School, Boston, MA, USA. Postdoctoral Research Fellow 04.2002-03.2005

Division of Clinical Bacteriology, IMPI. Huddinge University Hospital/Karolinska Institute, Stockholm, Sweden. Doctoral student/Teaching assistant 11.1996-03.2002

RESEARCH SKILLS:

Microbiology

Cultures of microorganisms (particularly those difficult to cultivate); microbial typing; transmission electronic microscopy on microbial morphology; antibiotic resistant analysis; bacterial extracellular secretory systems and their applications in protein expression; microbial gene regulation; identification of microbial flora.

Immunology

IVIAT (in vivo induced antigen technology to investigate microbial genes important for infections); antibody phage display for recombinant antibodies; ELISA; Western; phagocytosis assays; complement activation assay; ELISPOT; Cytometric Bead Array; Luminex for multiple cytokines profile; FACS; MACS for human cells isolation; viral neutralization assay; functional studies on molecules such as toxins and antibodies.

Molecular Biology

Sequencing based glycan profiling; lab-on-chip (Agilent, 2100 Bioanalyzor); large-scale plasmid preparation by Bio-Robot 9600; protein purification (affinity chromatography, AKTA FPLC, gel filtration); peptide purification (HPLC); Fluorescent Correlative Spectroscopy; confocal microscopy and live cell imaging; cytotoxicity assay; protein-protein interaction by Biacore; Genetic manipulations in prokaryotic and eukaryotic cells (plasmid construction for protein expression and for gene silencing, allele replacement, insert inactivation, and site direct mutagenesis for generating designed mutants); glyco-engineering on yeast (pathway manipulations); recombinant proteins produced by engineered yeast.

Molecular Diagnostics & Bioinformatics

Whole genome amplifications; LCR for mutation detection; Immuno PCR for toxins detection; quantitative real time PCR; large-scale gene screening by LightCycler480; gene scanning by qPCR using analysis software High Resolution Melting Master; genotyping on TB, HIV-1, HCV and HPV; microorganism typing based on ribosomal RNA, microbial genome data base from TIGR and Sanger, Ensembl, Map Viewer, ExPASy, PDB, microarray data analysis (GEPAS), EBI (Uniport, PRIDE, MSD, IntAct, Reactome).

email: lei.pei(at)idialog.eu